phase 4

Due to: 30 sep.  2017


Step 4 title: Optimization of biofungicide, treatment method and utility demonstration






1.Becze A., M. Roman, C. Puia, M. Dordai, D. Simedru, E. Luca, Comparative study regarding the total polyphenol content of some tinctures and oils from Romanian market, AGRICULTURA, AGRICULTURAL PRACTICE AND SCIENCE JOURNAL, 1-2 (101-102), 27-30, 2017, ISSN 1221-5317


2.Puia Carmen, Loredana Suciu, Raluca Miclea, 2017, Unconventional Bio-fungicides for the Seed Treatment Used in the Control of Main Wheat Diseases, ProEnvironment 10 (2017) 61- 67, Available online at http:// -


3.Miclea Raluca, Loredana Suciu, Carmen Puia, STUDY REGARDING THE EFFICACY OF UNCONVENTIONAL TREATMENT PRODUCTS AGAINST THE MAIN WHEAT KERNELS PATHOGENS, (AGR-2017-0028) – in the process of publishing in the  Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture.

(1) report  - Optimizing the biofungicide obtaining method-final

(2) report – Biofungicide optimization

(3)report- Treatment method optimization

(4) report- Demonstrating the utility and functionality of the product and the method

(5) technic and scientific report that highlights: optimization of the method of obtaining the biofungicide – activity finalization; biofungicide optimization; treatment method optimization for grain, utility and functionality demonstration of the biofungicide and the BIONEC treatment method


(1,2) The BIONEC fungicide is made by mixing different plant extracts with essential oils and a processed zeolite material (grinded, micronized, activated) treated with natural antifungal substances that are detained in the zeolite volcanic tuff structure. This with be used for grain treatment before sowing. The method of obtaining the BIONEC biofungicide consist in going through this steps:  ►documentation about the raw materials that will confirm the quality of the vegetal material, quality of the alcohol used for obtaining the hydro alcoholic extracts and the quality of the basic zeolite material ►hydro alcoholic extract obtaining, ►obtaining the essential oils ►obtaining the basic zeolite material for treatment effective realization  of the biofungicide


(3) Initial treatment method: immersing the grain seeds 3-5 minutes in biofungicide solutions, followed by grain treatment before sowing with the zeolite material (volcanic tuff), in accordance with the BIONEC biofungicide obtaining method. Proposed optimization: reducing the contact time between the biofungicide and the kernels by pulverization., followed by grain treatment with zeolite material (volcanic tuff), in accordance with the BIONEC biofungicide obtaining method. The efficiency of the proposed optimized treatment method was made.    

(4) The testing was made by using the breed called autumn grain ANDRADA and spring Pădureni. The result obtained in the field experiments showed that the optimal treatment method for the seed is pulverization. A meeting was scheduled at ICIA’s headquarter (15.09.2017), meeting where the project’s results and demonstration report were displayed. The testing of the BIONEC biofungicides in real field conditions was organized at Turda SCDA experimental field, on spring Pădureni breed and autumn ANDRADA. For determining the pathogenic attack level, the frequency (F%), intensity (I%) and attack grade (GA%) were  calculated using the formulas from the previous report. During the whole vegetation period, observations were conducted regarding sprouting, plant size, wheat ear length, foliar and wheat ear disease incidence. Productivity parameters were analyzed after harvesting along with weighing the production, determining the MMB and quality tests – protein, gluten and starch. The quality indicators were determined using NIR TANGO analyzer with BRUKER and INSTALB 600 accessories. The resulting data gathered form the observations were interpreted using the POLIFACT program. The observed pathogens from the vegetation time were Puccinia graminis – black rust, Puccinia recondita – brown rust, Zymoseptoria tritici .f.c. Septoria tritici and Phaeosphaeria nodorum f.c. S. nodorum – septoria leaf spot and Blumeria (Erysiphe) graminis f.c. Oidium monilioides – mildew.   The results regarding vegetation disease, production, and productivity elements for the ANDRADA and PĂDURENI breed, production and quality of production for ANDRADA and DURENI breed, wheat grains infested by fusarium showed the benefic action given by the BIONEC product after harvesting.

(5) The results were disseminated through 3 publications (2 already published and one ongoing)




Here you can download documentation of the project with number PN-II-PT-PCCA-2013-4-1857.



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USAMV (Universitatea de Siinte Agricole si Medicina Veterinara)

Calea Manastur 3-5, Cluj-Napoca 400372

Aula - Phythophathology

prof. Carmen Emilia Puia  ph.D

Tel: 0745-657-785

Fax: (+4)0264-593-792